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在Drosophila神经母细胞中微管体诱导的Pins/Galphai皮质极性
Sarah E Siegrist1, Chris Q Doe
1Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA.
Cell
|December 27, 2005
概括
微管和 Khc-73 和 大盘 (Dlg) 等特定蛋白质在多索菲拉神经细胞中建立皮质极性. 这一过程对于不对称的细胞分裂和产生细胞多样性至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 神经科学是一个神经科学.
背景情况:
- 皮层极性对于基本的细胞过程如分裂,迁移和分化至关重要.
- 虽然众所周知,微管在酵母中诱导皮质极性,但它们在元动物中的作用不太清楚.
- 神经母细胞中不对称的细胞分裂产生了细胞多样性,但皮层极化的潜在机制尚未完全阐明.
研究的目的:
- 研究微管在诱导Drosophila神经细胞中的皮质极性中的作用.
- 为了确定参与微管介导的皮质极化中的分子参与者.
- 了解这种途径如何与现有的机制协调,以确保适当的细胞分裂.
主要方法:
- 利用Drosophila神经细胞作为一个模型系统.
- 使用免疫光显微镜观察蛋白质定位.
- 进行共免疫沉试验以研究蛋白质相互作用.
- 研究了已识别的途径对细胞分裂和命运决定的功能后果.
主要成果:
- 证明了星际微管,基因素Khc-73,大盘 (Dlg) 诱导皮质极化Pins/Galphai在Drosophila神经母细胞.
- 显示这种皮质域对于螺旋轴不对称,子细胞大小不对称以及不同的兄弟姐妹命运至关重要.
- 确定Khc-73定位到微管加端,并与Dlg和Pins相互作用,这表明微管诱导极性的一种分子机制.
- 揭示了这种微管/Khc-73/Dlg通路与Inscuteable/Par通路并行运行,在细胞分裂过程中提供独特的时间和空间信息.
结论:
- 微管在元动物中对神经细胞皮质的两极分化起着至关重要的作用.
- 已识别的微管/Khc-73/Dlg通路是一种新的机制,有助于非对称的细胞分裂.
- 这条通路在转相期间协调皮质极性与线粒体旋轴,补充了Inscuteable/Par通路在预相中的作用.
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