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Updated: Jul 6, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

一个双参数FRET探针用于测量活细胞中的PKC和PKA活性

Justin Brumbaugh1, Andreas Schleifenbaum, Alexander Gasch

  • 1European Molecular Biology Institute (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Journal of the American Chemical Society
|January 5, 2006
PubMed
概括

研究人员开发了一种基于FRET的新型探针,用于同时监测活细胞中的蛋白激酶A (PKA) 和蛋白激酶C (PKC) 活性. 这种工具可以更深入地了解复杂的蜂信号网络.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...