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一种人工细胞表面受体的合成,使基胺亲和标签能够作为金属依赖的细胞透的功能

Siwarutt Boonyarattanakalin1, Sonalee Athavankar, Qi Sun

  • 1Department of Chemistry, The Pennsylvania State University, University Park, 16802, USA.

Journal of the American Chemical Society
|January 13, 2006
PubMed
概括

研究人员开发了一种合成细胞表面受体,使得具有胺标签的蛋白质能够进入细胞. 这种新方法显著提高了用于细胞生物学研究的蛋白质输送,而不会损害细胞.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...