与亚结合的T7促进剂,用于高效的转录光调节

Mingzhe Liu1, Hiroyuki Asanuma, Makoto Komiyama

  • 1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

概括

研究人员在T7促进体中使用亚博烯开发了可光控制的转录. 引入亚博烯允许光调节T7RNA聚合酶 (T7RNAP) 活动,提高转录效率.

相关概念视频

Transfer RNA Synthesis02:36

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Master Transcription Regulators02:23

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tRNA Activation02:26

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Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
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