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Light/dark Transition Test for Mice
Published on: November 13, 2006
恒定的黑暗是哺乳动物的昼夜代谢信号
Jianfa Zhang1, Krista Kaasik, Michael R Blackburn
1Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, Texas 77030, USA.
Nature
|January 20, 2006
概括
持续的黑暗会触发小鼠的昼夜节律,激活燃烧脂肪的基因,并通过升高的5'-AMP水平诱导类似冬眠的状态 ().
科学领域:
- 循环生物学的日间运行时间.
- 代谢调节 代谢调节 代谢调节
- 哺乳动物生理学 哺乳动物生理学
背景情况:
- 环境光线作为哺乳动物中昼夜节律的主要时间提供者.
- 在冬眠期间遇到的恒定黑暗,代表了一个自由运行的昼夜状态.
- 上神经核同步昼夜行为,其切除会破坏.
研究的目的:
- 阐明恒定黑暗调节脂肪代谢酶基因表达的机制.
- 为了确定关键的调解者参与昼夜调节的新陈代谢在不断的黑暗中.
- 调查昼夜时钟在和代谢变化中的作用.
主要方法:
- 小鼠被暴露在明暗 (LD) 和恒定暗 (DD) 周期中.
- 分析了小鼠前酶 (mClps) 和胰腺脂酶相关蛋白2 (mPlrp2) 的基因表达.
- 在血液中测量了5eal-AMP,葡萄糖和非化脂肪酸的水平.
- 随着5次eal-AMP的使用,Torpor被诱导和分析.
主要成果:
- 在DD,但不是LD周期下,小鼠前酶 (mClps) 和胰腺脂酶相关蛋白2 (mPlrp2) 基因表达显示了周围器官的昼夜激活.
- 循环节缺陷小鼠表现出这些基因的放松表达.
- 在DD小鼠中血中5eal-AMP升高被确定为一个关键的调解者,诱导LD动物的和mClps表达.
- 由代谢压力引起的丧与增加的5eal-AMP水平相关.
- 在DD和LD条件之间,血糖和非化脂肪酸水平发生逆转,5eal-AMP与葡萄糖水平相互关联.
结论:
- 持续的黑暗会激活哺乳动物的昼夜新陈代谢节奏,调节脂肪的代谢.
- 5eal-AMP是一个关键的调解器,将昼夜信号与和代谢调整联系起来.
- 昼夜时钟在协调对长时间黑暗的代谢反应方面发挥着至关重要的作用,可能有助于冬眠.
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