概括
禽类白血病病毒 (ALV) 含有独特的基因组序列,在正常中没有发现,与一些肉 Sarcoma 病毒有显著差异. 这些ALV序列出现在Fabricius的囊中感染后,在瘤发育之前.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 禽类白血病病毒 (ALV) 是一种致癌逆转录病毒,在家禽养殖中造成重大经济损失.
- 了解外源ALV和内源病毒序列之间的基因组关系对于疾病控制至关重要.
- 以前的研究表明,ALV和细胞DNA之间存在共同和独特的序列.
研究的目的:
- 为了研究最近鸟类白血病病毒现场分离的基因组序列.
- 将这些序列与内源性病毒DNA和其他禽类逆转录病毒进行比较.
- 确定正常和瘤细胞中ALV特异序列的存在和重要性,以及它们在感染期间的出现.
主要方法:
- 在DNA过剩条件下的DNA-RNA杂交被用来比较病毒RNA基因组与细胞DNA.
- 竞争性杂交分析被用来比较不同的禽类逆转录病毒菌株.
- 在Fabricius感染后的囊中检测ALV特异序列.
主要成果:
- 至少18%的ALV基因组序列在正常的DNA中没有被检测到,这表明外源起源.
- 大多数共享的ALV序列与内源RAV-0原病毒杂交.
- 亚LV基因组的外源部分与两种肉瘤病毒 (Pr-RSV-C和B-77) 有显著差异.
- 增加ALVRNA与淋巴瘤DNA的杂交,与感染时间和Fabricius毛囊中的瘤发育相关,其中一半的增加是在可见转化之前.
结论:
- 最近的ALV野外分离物具有独特的基因组序列,与内源性病毒DNA不同.
- 这些外源ALV序列与某些肉瘤病毒的序列有所不同.
- 在感染后在Fabricius的毛囊中可以检测到ALV特定的序列,并且与瘤转变有关,在瘤的组织学证据之前出现.
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