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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events10:59

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Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation...
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This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are...
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Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
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相关实验视频

Updated: Jan 29, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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孕诺能稳定微管,促进斑马鱼胚胎细胞的运动.

Hwei-Jan Hsu1, Ming-Ren Liang, Chao-Tsen Chen

  • 1Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.

Nature
|January 27, 2006
PubMed
概括
此摘要是机器生成的。

孕诺通过维持微管稳定性,促进胚胎细胞在表皮质期的运动. 这种类固醇激素对于斑马鱼胚胎的正确形态发生和身体形状发育至关重要.

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科学领域:

  • 发育生物学是发展生物学.
  • 细胞生物学 细胞生物学
  • 内分泌学 在内分泌学.

背景情况:

  • 胚胎细胞运动对于形态发生至关重要.
  • 调节胚胎细胞迁移的精确机制尚未完全理解.
  • 类固醇激素在生物过程中扮演着不同的角色.

研究的目的:

  • 为了调查pregnenolone在胚胎细胞迁移中的作用.
  • 阐明孕醇影响细胞运动的分子机制.

主要方法:

  • 使用斑马鱼作为模型生物.
  • 采用反感性摩尔福利诺寡核酸来减少cyp11a1的表达.
  • 用于救援实验的cyp11a1RNA和孕.
  • 评估了微管的聚合水平.

主要成果:

  • 减少cyp11a1表达导致斑马鱼胚胎的延迟表.
  • 这种延迟被cyp11a1RNA或孕类补充剂部分挽救.
  • 发作迟缓与微管聚合率降低相关.
  • 孕醇治疗恢复了微管的丰富性.

结论:

  • 孕诺在表皮质期促进细胞迁移.
  • 孕醇保持了微管的稳定性,促进了胚胎细胞的运动.
  • 这突显了孕在发育过程中的新作用.