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Methylated DNA Immunoprecipitation
Published on: January 2, 2009
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DEMETER DNA 糖酶通过异位基因特异性脱甲基化来建立MEDEA多组基因的自我印记
Mary Gehring1, Jin Hoe Huh, Tzung-Fu Hsieh
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|February 14, 2006
概括
阿拉比多普西斯MEDEA (MEA) 印记是通过DEMETER (DME) 消除DNA甲基化来激活母性等位基因而建立的. 父亲的沉默是由母亲的MEA维持的,而不是DNA甲基化.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 阿拉比多普西斯塔利亚纳研究研究
背景情况:
- 梅迪亚 (MEA) 是阿拉比多普西斯内中印制的Polycomb组基因,具有母体表达和父体沉默.
- 通过DEMETER (DME) 和METYLTRANSFERASE I (MET1) 调节的DNA甲基化与MEA印记有关.
- 控制MEA的等位基特异性表达和沉默的精确机制尚未完全阐明.
研究的目的:
- 调查DEMETER (DME) 在MEA位点建立母性基因特异性低甲基化中的作用.
- 确定MEA的父性等位基沉默背后的机制.
- 为了澄清MEA印记中DNA甲基化和Polycomb组蛋白之间的相互作用.
主要方法:
- 在体外生化测试以评估DME对5-甲基细胞因子的DNA糖酶活性.
- 在大肠杆菌中表达DME以研究其酶功能.
- 分析DNA甲基化模式和基因表达在阿拉比多的内突变.
主要成果:
- 德米特 (DME) 负责在内精中对MEA基因进行母性基因特异性低甲基化.
- DME在体外和大肠杆菌中切除5-甲基细胞素,其中基底部位可能会防止DNA断裂.
- 父亲的MEA沉默独立于DNA甲基化,并由母体的Polycomb组蛋白控制,包括MEA本身.
结论:
- 德米特 (DME) 通过去甲基化母性等位基来启动MEA印记,从而导致其激活.
- 母亲MEA,一个Polycomb组蛋白质,积极沉默父系MEA等位基因,保持印记在内.
- 这项研究揭示了一种新的表观遗传机制,其中DNA脱甲基和Polycomb介导的沉默合作建立和维持印记.
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