一类膜蛋白质,塑造管状内等质网膜
Gia K Voeltz1, William A Prinz, Yoko Shibata
1Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Cell
|February 14, 2006
概括
网膜和DP1/Yop1p是必不可少的不可分割的膜蛋白质,通过稳定其管状网络来塑造内质网膜 (ER). 它们的缺席破坏了ER结构,突出了它们在膜形态发生中的作用.
科学领域:
- 细胞生物学 细胞生物学
- 膜生物学 膜生物学
- 生物化学 生物化学
背景情况:
- 细胞内膜网膜 (ER) 的特有的管状形状对其功能至关重要.
- 产生和维护ER管网的机制尚未完全理解.
研究的目的:
- 为了研究构成和维护ER管状网络的分子机制.
- 为了确定参与塑造ER膜管道的关键蛋白质.
主要方法:
- 利用体外系统研究ER网络形成.
- 使用生化和遗传方法研究了网膜蛋白 (Rtn4a/NogoA) 和DP1/Yop1p的作用.
- 在酵母 (S. cerevisiae) 和哺乳动物细胞中检查了蛋白质的局部化和功能.
主要成果:
- 在体外 (in vitro) 形成ER网络所需的是整体膜蛋白,网膜蛋白 (Rtn4a/NogoA) 和DP1/Yop1p.
- 这些蛋白质主要局限于管状ER,不包括它们在ER片中.
- 网膜的过度表达会导致管状膜结构.
- 在酵母中同时缺少网膜和Yop1p会导致管状ER受损.
结论:
- 网球和DP1/Yop1p充当"形态"蛋白质,稳定高度曲的ER膜管道.
- 这些蛋白质对于产生和维护内等离子体网膜特有的管状结构至关重要.
相关概念视频
Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...


