希斯H4-K16乙化控制着染色质结构和蛋白质相互作用
Michael Shogren-Knaak1, Haruhiko Ishii, Jian-Min Sun
1Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Science (New York, N.Y.)
|February 14, 2006
概括
在 lysine 16 (H4-K16Ac) 中对 histone H4 的乙化破坏了染色体纤维的形成和重塑. 这一单一的修改影响了更高阶的染色质结构和蛋白质相互作用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基斯修饰对于调节染色体结构和功能至关重要.
- 在lysine 16 (H4-K16Ac) 上对基因组H4的乙化是真核生物中一个关键的翻译后修饰.
- 了解H4-K16Ac在染色体组织中的精确作用至关重要.
研究的目的:
- 研究同质H4-K16Ac.Ac. 的结构和功能后果.
- 为了阐明这种特定的组素标记如何影响更高阶染色体组合.
- 为了确定H4-K16Ac对染色体重塑酶活性的影响.
主要方法:
- 利用原生化学结合合成同质的H4-K16Ac.Ac.
- 纳入基因组组合组合的基因组组合组合.
- 评估了对30纳米纤维形成和跨纤维相互作用的影响.
- 研究了对ACF酶调动单核细胞体的能力的影响.
主要成果:
- 同质的H4-K16Ac抑制了紧的30纳米长的色素纤维的形成.
- 这种修改阻碍了染色质的跨纤维相互作用能力.
- H4-K16Ac显著降低了单核细胞上ACF重塑酶的活性.
结论:
- H4-K16Ac在调节高阶染色体结构方面发挥着至关重要的作用.
- 这种单基因组修饰会影响染色蛋白和非基因组蛋白之间的功能相互作用.
- H4-K16Ac提供了对染色体组织和功能的调节机制的见解.
相关概念视频
02:15Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
02:21Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...