在体内可视化胚胎干细胞存活,增殖和心脏分娩后的迁移
Feng Cao1, Shuan Lin, Xiaoyan Xie
1Department of Radiology, Bio-X Program, Stanford University School of Medicine, Stanford, CA 94305-5344, USA.
Circulation
|February 16, 2006
概括
这项研究开发了一种新的三重融合记者基因,用于在体内跟踪干细胞. 报告员系统使胚胎干细胞生存和增殖的非侵入性监测成为可能,这对于推进干细胞疗法至关重要.
科学领域:
- 再生医学是一种再生医学.
- 生物技术是生物技术.
- 医疗成像医学成像
背景情况:
- 干细胞疗法对组织再生具有前景.
- 在体内对干细胞的监测是当前方法的挑战.
研究的目的:
- 开发和描述一种新的三重融合记者基因,用于体内干细胞跟踪.
- 评估记者基因对胚胎干细胞功能的影响.
- 评估报告员系统对监测干细胞存活和增殖的有用性.
主要方法:
- 用一种编码三重融合 (TF) 记者基因 (火 luciferase,单体红色光蛋白,截断的胺基酶) 的lentiviral 载体转化小鼠胚胎干细胞 (ES).
- 评估了报告者基因表达及其对ES细胞活力,增殖和分化的影响.
- 将ES-TF细胞注射到裸体大鼠的心肌中,并使用生物发光和正子发射断层扫描 (PET) 图像进行监测.
- 评估了瘤的形成,并评估了甘西克洛维尔治疗对瘤发展的影响.
主要成果:
- ES细胞活力,增殖和分化不受TF记者基因的影响.
- 生物发光和PET信号在体内证实了ES细胞的生存和增殖.
- 组织学分析显示了瘤的形成,甘西克洛维尔治疗可以预防这种形成.
结论:
- 这项研究首次描述了稳定表达光,生物发光和PET记者基因的ES细胞.
- 开发的成像平台允许对干细胞动力学的非侵入性监测.
- 这种多功能系统在干细胞治疗研究和临床应用方面具有重大潜力.
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