直接重复作为对甲状腺激素,视网酸和维生素D3受体的选择性反应元素
K Umesono1, K K Murakami, C C Thompson
1Gene Expression Laboratory, Howard Hughes Medical Institute, Salk Institute for Biological Studies, San Diego, California 92186-5800.
Cell
|June 28, 1991
概括
直接重复的甲状腺激素反应元素 (TREs) 与平行体TREs不同. 半位点间距关键地决定了选择性的激素反应,影响了甲状腺激素,视网酸和维生素D3的信号传递.
科学领域:
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
- 遗传学 是一个遗传学.
背景情况:
- 甲状腺激素反应元素 (TREs) 调节基因表达.
- TREs的结构,特别是半站式布局,影响受体结合和下游信号传输.
- 了解TRE结构的细微差别对于破译激素调节至关重要.
研究的目的:
- 识别和描述具有直接重复半站点安排的新型TRE.
- 调查半站点间距对TRE功能和选择性的影响.
- 阐明DNA序列和间距在差异激素受体结合中的作用.
主要方法:
- 直接重复的TRE的识别.
- 功能性测试以评估对甲状腺激素 (T3),视网酸和维生素D的反应3.
- 与观察到的荷尔蒙反应的DNA结合亲和关系的相关性.
主要成果:
- 直接重复的TRE被确定和描述.
- 与palindromic TREs不同,直接重复的TREs没有产生视网膜酸反应.
- 用单个核酸改变半位点间隔将TREs转化为网膜酸或维生素D3反应元素,同时失去T3反应.
- 结果与各自激素受体的DNA结合亲和关系相关.
结论:
- 双重重复激素反应元素在生物调节中通常很重要.
- 在TREs中,半位点间距作为实现选择性荷尔蒙反应的关键代码.
- 这一发现对理解内分泌信号通路和开发向治疗有意义.
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