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相关概念视频

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
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相关实验视频

Updated: Jul 8, 2026

Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
11:49

Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)

Published on: January 29, 2009

氨酸蛋白激酶介导神经生长因子和神经热素-3的线索性特性.

C Cordon-Cardo1, P Tapley, S Q Jing

  • 1Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.

Cell
|July 12, 1991
PubMed
概括

神经生长因子 (NGF) 作为一种强有力的辅激素,通过gp140trk受体驱动细胞增殖和转化. 这种受体还结合神经热素-3 (NT-3),调解不同的细胞反应.

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Last Updated: Jul 8, 2026

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11:49

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Published on: January 29, 2009

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09:46

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Published on: November 21, 2012

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 神经科学是一个神经科学.

背景情况:

  • trk原型瘤基因产物gp140trk是神经生长因子 (NGF) 的受体.
  • NGF信号传递对神经元发育和生存至关重要.
  • gp140trk在线粒发生的作用需要进一步阐明.

研究的目的:

  • 为了研究NGF的真菌生成潜力及其对gp140trk受体的依赖性.
  • 为了确定gp140trk是否调解对其他神经营养素的反应.
  • 通过gp140trk探索NGF和NT-3通过gp140trk激活的信号通路.

主要方法:

  • 使用了表达gp140trk受体的NIH 3T3细胞.
  • 评估了线粒体发生反应,包括在半固体介质中进入S阶段和生长.
  • 研究了对NGF,NT-3和BDNF的受体结合特异性.
  • 监测gp140trk的酸化和c-Fos的表达.

主要成果:

  • NGF是一种强大的线粒激素,诱导NIH 3T3细胞的增殖和形态转变.
  • 这些NGF诱导的效应依赖于gp140trk,而不是低亲和度NGF受体.
  • gp140trk与NT-3结合,但不与BDNF结合.
  • 无论NGF和NT-3都会触发gp140trk酸化和c-Fos表达,而NT-3则显示出较少的线索性活性.

结论:

  • gp140trk受体足以在增殖细胞中调解NGF和NT-3信号传导.
  • gp140trk在NGF诱导的线粒生成中起着至关重要的作用.
  • 在gp140trk下游的差异信号可能解释了对NGF和NT-3的不同反应.