相关实验视频

Updated: Jul 7, 2026

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes

Published on: December 15, 2015

测试使用合成循环二聚体合成的被动膜透性的构造假设

Taha Rezai¹, Bin Yu, Glenn L Millhauser

¹Department of Chemistry and Biochemistry, University of California, Santa Cruz, 95064, USA.

Journal of the American Chemical Society

|February 24, 2006

概括

分子构造显著影响被动膜扩散. 循环酸中的分子内键增强了膜的透性,其中一个二聚体呈现的速率与环素A相似.

科学领域:

生物化学生物化学
物理化学物理化学
药理学药理学是指药理学的学科.

背景情况:

对小分子被动膜扩散的 conformational 效应仍然不太了解.
假设分子内键,特别是涉及胺N-H组,可以降低溶解成本并增强扩散.
研究循环二聚体可以阐明构造在膜运输中的作用.

研究的目的:

为了测试内部分子键影响被动膜扩散速率的假设.
描述不同形状的循环二聚体的膜扩散.
为了将形状性质与观察到的扩散率相关联.

主要方法:

基于cyclo[Leu-Leu-Leu-Leu-Pro-Tyr]的循环六二聚体的合成和表征.
测量已识别的双立体体的被动膜扩散速率.
溶液核磁共振 (NMR) 研究和/ (H/D) 交换实验,以评估分子内键和动态.

主要成果:

两个循环六二聚体在被动膜扩散速率上表现出2个日志单位的差异.
在膜扩散率和分子内键的程度之间观察到强烈的相关性.
更快的H/D交换率与更高的膜透性相关.

结论:

形状,特别是分子内结的程度,是小分子被动膜扩散的关键决定因素.
最透的二聚体,cyclo[d-Leu-d-Leu-d-Leu-Pro-Tyr],表现出与口服药物cyclosporine A.相比的被动膜扩散.
这项研究为改善膜传输的分子设计提供了宝贵的见解,这与药物开发有关.

更多相关视频

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Published on: May 1, 2020

相关概念视频

Membrane Fluidity

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...

Multi-pass Transmembrane Proteins and β-barrels

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...