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Updated: Jul 18, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
07:31

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Published on: March 28, 2014

刚性,特异性和离散的金纳米粒子/抗体结合物

Christopher J Ackerson1, Pablo D Jadzinsky, Grant J Jensen

  • 1Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.

Journal of the American Chemical Society
|February 24, 2006
PubMed
概括

研究人员开发了一种方法,使用黄金集群精确标记蛋白质. 这种技术将黄金集群与工程抗体碎片结合在一起,使特定的蛋白质准和可视化成为可能,正如流感神经aminidase所示.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.