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相关概念视频

Convergent Evolution01:54

Convergent Evolution

Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

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相关实验视频

Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

设计的酶功能的不同演变.

Yasuo Yoshikuni1, Thomas E Ferrin, Jay D Keasling

  • 1UCSF/UCB Joint Graduate Group in Bioengineering, Lawrence Berkeley National Laboratory, Berkeley, California 94710, USA.

Nature
|February 24, 2006
PubMed
概括
此摘要是机器生成的。

研究人员通过了解蛋白质可塑性来设计新的酶. 这项研究通过修改合成酶中的可塑性残留物来证明合理的酶设计,从而产生特定且活性的生物催化剂.

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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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相关实验视频

Last Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

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Published on: March 25, 2020

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 酵素工程是什么? 酶工程是什么

背景情况:

  • 具有杂乱功能的蛋白质通过微小的氨基酸变化 (可塑性) 进化出更高的特异性和活性.
  • 了解这种分离的分子进化可以指导设计更具体和更活跃的酶.
  • 活跃地点的塑性残留对于功能适应性至关重要.

研究的目的:

  • 为了研究塑性残留如何对分子进化作出贡献.
  • 为酶工程制定一个合理的设计方法.
  • 构建具有改变催化功能的新型合成酶.

主要方法:

  • 在散乱的玛-木烯合成酶的活性位点内探测了可塑性残留物.
  • 使用数学模型系统地重组识别的可塑性残留物.
  • 构建和描述了新的烯合成酶.

主要成果:

  • 成功设计了七种特定且活跃的烯合成酶.
  • 这些新型酶利用不同的反应途径产生不同的酸产品.
  • 证明了利用蛋白质支架进化的可行性.

结论:

  • 基于可塑性的合理酶设计方法是有效的.
  • 这项研究提供了证据,证明了在酶工程中理解分子进化的实用性.
  • 突出了创建具有所需特异性和活动的定制酶的潜力.