肌源性HLH蛋白的功能活性需要在体内与E12/E47类蛋白进行异质寡合化
A B Lassar1, R L Davis, W E Wright
1Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|July 26, 1991
概括
类似E12/E47的蛋白质与体内肌原蛋白MyoD和肌原蛋白相互作用. 这种相互作用对于肌肉分化和调节下游肌肉基因表达至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 肌原螺旋环螺旋 (HLH) 蛋白质,如MyoD和肌原蛋白,是肌肉分化的关键调节者.
- 涉及这些蛋白质的精确相互作用和调节机制对于理解肌肉发育至关重要.
研究的目的:
- 提供E12/E47类蛋白和肌源性HLH蛋白 (MyoD,myogenin) 之间的体内相互作用的证据.
- 阐明这些相互作用对肌肉基因转录和分化的功能后果.
主要方法:
- 在COS细胞中对共传染进行测试,以评估转录协同作用.
- 用特定的抗菌剂进行电泳运动转移测定 (EMSA) 来识别蛋白质复合体.
- 对蛋白质酸化状态的分析.
- 评估改变E2A表达的细胞中的肌肉分化.
主要成果:
- 通过MyoD和E47.7证明了协同的转录增强.
- 在MEF1部位中发现含有MyoD或myogenin与E12/E47类蛋白相关的蛋白质复合物.
- 表明E47关联改变了MyoD酸化.
- 在抑制E2A活动的细胞中观察到肌肉分化减少.
结论:
- 类似E12/E47的蛋白质在体内与MyoD和myogenin进行物理相互作用.
- 这些相互作用对于激活下游肌肉调节器和促进终端肌肉分化至关重要.
- 与E12/E47类蛋白质复合的MyoD作为肌肉发育中的中心调节点.
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