在缺乏低亲和性NGF受体的纤维细胞中,TrkB调解了BDNF/NT-3依赖的生存和繁殖
David J Glass1, Steven H Nye, Petros Hantzopoulos
1Regeneron Pharmaceuticals, Inc., Tarrytown, New York 10591.
Cell
|July 26, 1991
概括
大脑衍生神经营养因子 (BDNF) 和神经营养因子-3 (NT-3) 通过TrkB信号传递促进细胞存活和增殖. 低亲和度NGF受体 (LNGFR) 对这些神经质蛋白反应并不必不可少.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 神经营养素,包括神经生长因子 (NGF),脑衍生神经营养因子 (BDNF),神经营养素-3 (NT-3) 和神经营养素-4 (NT-4),对于神经元的生存至关重要.
- 这些因素通过受体氨酸激酶的Trk家族发出信号,可能与低亲和度NGF受体 (LNGFR) 相结合.
研究的目的:
- 研究TrkB在调解细胞对BDNF和NT-3反应中的作用.
- 为了确定LNGFR在神经营养素信号传递中的必要性.
主要方法:
- 在NIH 3T3纤维细胞中表达TrkB.
- 对BDNF和NT-3的反应中细胞存活和增殖的评估.
- 对剂量依赖反应的分析和与初级神经元存活率的比较.
主要成果:
- 纤维细胞中的TrkB表达赋予了对BDNF和NT-3的生存和增殖反应,而BDNF是一个更强大的配体.
- 在TrkB表达纤维细胞中BDNF的剂量反应曲线与初级神经元存活率观察到的情况相似.
- 纤维细胞系统中LNGFR的缺失表明,它对生理神经质蛋白信号传递并不重要.
结论:
- TrkB是BDNF和NT-3下游的生存和扩散信号的关键媒介.
- 在生理度下,LNGFR对于调解神经营养素的影响并非不可或缺.
- 这些发现提供了对神经营养素信号通路和神经元细胞死亡机制的洞察.
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