收回:三角体反应元素的非编码RNA招募了Drosophila Ash1到Ultrabithorax
Tilman Sanchez-Elsner1, Dawei Gou, Elisabeth Kremmer
1Department of Biochemistry, University of California, Riverside, CA 92521, USA.
Science (New York, N.Y.)
|February 25, 2006
概括
来自trithorax响应元素 (TREs) 的非编码转录会招募Ash1表观遗传调节器来激活基因表达. 这种机制对于像Ultrabithorax (Ubx) 这样的Drosophila同源基因的组织特异性转录至关重要.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
- 基因规则 基因规则
背景情况:
- 同源性基因由称为三体反应元件 (TREs) 的 cis-调节元件调节.
- TREs是已知的表观遗传激活剂的标,并以特定组织的方式转录.
- 在基因激活中TRE转录的确切功能仍然不清楚.
研究的目的:
- 调查TRE转录在Drosophila同源基因Ultrabithorax (Ubx) 的表观遗传激活中的作用.
- 通过招募表观遗传调节者来确定TRE转录如何调解转录激活.
主要方法:
- 对Drosophila中的TRE转录和Ash1招募的分析.
- 生物化学试验研究Ash1的SET域与TRE转录的结合.
- 转基因表达TRE转录以评估救援效应.
- 小干扰RNA (siRNA) 介导的TRE转录的降解,以评估功能影响.
主要成果:
- 在Ubx基因中的三个特定TREs的转录将表观遗传调节器Ash1招募到模板TREs中.
- 在Drosophila中,TRE转录和Ash1招募与Ubx转录同时发生.
- Ash1的SET域与TRE转录结合,具有特定的杂交,导致对色素中对应的TREs的有针对性的招募.
- 在缺乏内源转录的细胞中,在TRE转录的转基因转录时观察到Ubx表达的恢复.
- 使用siRNA降低了Ash1的招募和Ubx的表达.
结论:
- 非编码的TRE转录对于基因表达的表观遗传激活至关重要.
- TRE转录作为关键的中间体,招募像Ash1这样的表观遗传调节器到特定的基因组位置.
- 这种机制在确保同源性基因的正确组织特异性表达方面发挥着重要作用.
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