氨基酸的运输由老鼠的生态热带逆转录病毒受体
J W Kim1, E I Closs, L M Albritton
1Howard Hughes Medical Institute, Boston, Massachusetts.
Nature
|August 22, 1991
概括
鼠类白血病病毒 (MuLV) 受体被确定为氨基酸载体. 这一发现揭示了这些病毒受体的新型细胞功能,影响了细胞生物学和病毒学.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 对生态型小鼠白血病病毒 (MuLV) 的敏感性取决于特定的膜受体.
- 受感染的细胞产生包膜蛋白 (gp70),它与该受体结合,阻止进一步的感染.
- MuLV受体蛋白的细胞功能在很大程度上是未知的.
研究的目的:
- 为了研究MuLV受体蛋白的细胞功能.
- 为了确定MuLV受体与其他已知的蛋白质之间的潜在相似性.
- 探索MuLV受体与病毒结合的功能后果.
主要方法:
- 基于计算机的MuLV受体与GenBank数据库的序列比较.
- 分析MuLV受体和相关蛋白质中的膜跨域.
- 在信使RNA注射后研究受体功能的Xenopus卵细胞表达系统.
主要成果:
- MuLV受体在其覆盖膜的域中与Saccharomyces cerevisiae中的阿尔金因和丁透酶具有结构上的相似性.
- 表达MuLV受体的Xenopus卵细胞表现出氨基酸 (L-氨酸,L-lysine,L-ornithine) 的吸收增加.
- MuLV受体的运输特征和表达模式与哺乳动物氨基酸载体y+的已知特性一致.
结论:
- MuLV受体蛋白作为一个氨基酸转运器.
- 这一发现为病毒受体提供了一个新的细胞作用.
- 这项研究将病毒进入机制与基本的细胞运输过程联系起来.
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