相关实验视频

Updated: May 6, 2026

Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks
08:14

Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks

Published on: May 24, 2014

18.1K

哺乳动物的性别染色体专业化和退化

Jennifer A Marshall Graves1

  • 1Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia. jenny.graves@anu.edu.au

Cell
|March 15, 2006
PubMed
概括

人类的性别染色体,特别是Y染色体,具有独特的结构和基因内容,挑战了典型的基因组规则. 基因组数据表明,Y染色体可能正在退化,并因其进化路径而走向灭绝.

更多相关视频

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR10:16

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

9.8K
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

11.4K

相关概念视频

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
26.4K
Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
9.2K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
14.5K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K