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相关概念视频

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

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This protocol provides a method for the systematic global optimization of genetically encoded biosensors through automation-assisted genetic library generation and assessment. This is coupled with design-of-experiment methodologies to streamline experimentation and enable the selection of genetic components to tune biosensors to specific design outcomes.
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Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical...
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Field-effect biosensing (FEB) is a label-free technique for detecting biomolecular interactions. It measures the electric current through the graphene biosensor to which the binding targets are immobilized. The FEB technology was used to evaluate biomolecular interactions between Hsp90 and Cdc37 and a strong interaction between the two proteins was...
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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

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This study aimed to present a strategy for identifying drug-peptide interactions. The strategy involves the biopanning of drug-recognizing short peptides based on a quartz-crystal microbalance (QCM) biosensor, followed by bioinformatics analysis for quantitatively assessing the information obtained for the drug recognition and annotation of the drug-binding sites on...
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Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

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The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold...
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BioMEMS and Cellular Biology: Perspectives and Applications16:30

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The ability to culture cells has revolutionized hypothesis testing in basic cell and molecular biology research. It has become a standard methodology in ...
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相关实验视频

Updated: Jan 6, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

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CSL-Notch-Mastermind三元复合物的晶体结构与DNA结合在一起.

Jeffrey J Wilson1, Rhett A Kovall

  • 1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.

Cell
|March 15, 2006
PubMed
概括

这项研究揭示了关键的Notch信号复合体的结构. 这一发现解释了CSL蛋白如何在发育过程中从抑制转录转换为激活基因转录.

科学领域:

  • 分子生物学分子生物学
  • 发展生物学 发展生物学
  • 结构生物学 结构生物学

背景情况:

  • 痕信号对于细胞通信,胚胎模式和发育至关重要.
  • CSL (CBF1/Su(H) /Lag-1) 是一种转录因子,通过与共调剂相互作用来调节Notch目标基因.
  • 转录激活涉及从CSL通过Notch细胞内域 (NotchIC) 取代核心压缩剂,并招募大脑协同激活剂.

研究的目的:

  • 确定由CSL,NotchIC和大脑与DNA结合而形成的三元复合体的结构.
  • 为了阐明基底的分子机制口介导的转录激活.

主要方法:

  • 使用X射线晶体学来确定三元复合体的3.1 Å结构.
  • 在复合体内的蛋白质-蛋白质相互作用的分析.

主要成果:

  • 正如预测的那样,Notch的RAM域与CSL的β三叶子域相互作用.
  • CSL的C端域在与Notch ankyrin重复和Mastermind的接口中扮演着关键的,意想不到的角色.
  • 三元复合物的形成会导致CSL的显著构造变化.

结论:

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  • 该结构为CSL如何从抑制剂转变为激活剂提供了分子基础.
  • 突出了CSL C终端域在调解转录激活相互作用中的关键作用.
  • 提供了关于Notch信号通路对基因表达的调节的见解.