烟草马赛克病毒的移动蛋白质改变了等离子体体大小的排除限制
1Botany Department, University of California, Davis, CA 95616, USA.
Science (New York, N.Y.)
|October 20, 1989
概括
烟草马赛克病毒运动蛋白 (MP) 通过增加等离子体的大小排除极限来促进病毒的传播. 这使得更大的分子,如光德克斯,可以在植物细胞之间移动.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 烟草马赛克病毒 (TMV) 的30千多运动蛋白 (MP) 对于植物细胞之间的病毒后代运动至关重要.
- 要了解MP的作用机制,需要研究其对等离子体功能的影响.
研究的目的:
- 为了研究TMV运动蛋白对等离子体大小排除极限 (SEL) 的影响.
- 为了确定MP是否直接改变等离子体的物质透性.
主要方法:
- 利用一种新的方法,将非等离子体透的光探针送入烟草叶海绵状半细胞的细胞质中.
- 采用了表达TMV MP基因的转基因烟草植物和对照植物.
- 追踪了具有分子质量为9400达尔顿的光素异硫酸标记的德克斯 (F-德克斯) 在细胞之间的运动.
主要成果:
- 光德克斯 (9400达尔顿) 在表达TMV MP.的转基因植物细胞之间成功移动.
- 对照工厂的大小排除极限明显较低,在700至800达尔顿之间.
- 在叶子中没有观察到F-dextran代谢的证据,证实了探针的完整性.
结论:
- 烟草马赛克病毒运动蛋白直接增强了等离子体的功能.
- MP显著增加了等离子体的大小排除极限,促进了更大的分子运输.
- 这项研究提供了MP在调节植物细胞间通信通路中的作用的直接证据.
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