一个发育调节的骨肌肉化物通道的主要结构和功能表达
K Steinmeyer1, C Ortland, T J Jentsch
1Centre for Molecular Neurobiology (ZMNH), Hamburg University, Germany.
Nature
|November 28, 1991
概括
研究人员在老鼠骨肌中确定了一个关键的化物通道. 这一发现对于了解肌肉膜的稳定性和诸如肌肉等疾病至关重要.
科学领域:
- 身体生理学 身体生理学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 骨肌肉在很大程度上依赖于化物离子来保持膜电位的稳定性.
- 化物导电率的破坏,例如由9-甲-碳酸,导致肌.
- 鱼类的电器官,来自肌肉,表达化物通道.
研究的目的:
- 为了克隆编码大鼠骨肌肉化物通道的互补DNA (cDNA).
- 为了研究它与Torpedo marmorata的同质性,我们研究了电平的化物通道.
- 证实其在骨肌功能和肌的作用.
主要方法:
- 使用Torpedo electroplax 化物通道序列进行同质选.
- 克隆大鼠骨肌的化通道cDNA.
- 在Xenopus卵细胞中道的表达,用于功能分析.
- 在产后发育期间分析信使RNA (mRNA) 水平.
主要成果:
- 鉴定出了一种同类于鱼雷通道的994氨基酸蛋白.
- 鼠标频道与鱼雷频道共享大约54%的身份.
- 在骨肌肉中观察到主要的表达,mRNA水平在出生后增加.
- 卵子细胞中的功能表达产生了对9-甲-碳素酸敏感的电流.
结论:
- 克隆道很可能是大鼠骨肌肉的主要化通道.
- 这一发现为骨肌的化物导电性和肌性提供了分子基础.
- 道在膜电位稳定中的作用通过功能研究得到证实.
更多相关视频
相关概念视频
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Bone Matrix
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
Smooth Muscle Contraction
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...


