Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Smooth Muscle Contraction01:25

Smooth Muscle Contraction

Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors01:28

Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors

Phosphodiesterase 5 (PDE5) inhibitors are potent enzymes that function to hydrolyze cyclic nucleotides to their corresponding 5' monophosphates. Their unique biochemical properties have been applied in treating Pulmonary Arterial Hypertension (PAH).
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists01:18

Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists

Endothelins (ETs) are potent vasoactive peptides critical in the human body's various physiological and pathological processes. One of the most promising therapeutic strategies for treating pulmonary arterial hypertension (PAH) involves counteracting the effects of these endothelins using a class of drugs known as endothelin receptor antagonists.
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme (ECE). Of...
Hypertension II: Pathophysiology01:29

Hypertension II: Pathophysiology

Hypertension is a chronic condition in which the blood's force against artery walls is excessively high, posing risks such as heart disease. The condition's underlying mechanisms involve complex interactions among the cardiovascular, kidney, and autonomic nervous systems.Renin-Angiotensin-Aldosterone System (RAAS): This system significantly influences blood pressure regulation. When blood pressure decreases, the kidneys secrete renin. This enzyme transforms angiotensinogen, a plasma protein,...
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Placental Growth Factor Promotes Endothelial Activation and Inflammatory Remodelling in Pulmonary Hypertension.

Cardiovascular research·2026
Same author

Sotatercept reduces bone morphogenetic protein signaling in patients with pulmonary arterial hypertension.

Science translational medicine·2026
Same author

Impact of Pulmonary Arterial Hypertension Therapies on Gas Exchange in Portopulmonary Hypertension.

Chest·2026
Same author

Bile Acid Alteration and Alveolar Dysfunction in Cirrhosis: An Aggravating Factor or a New Piece in the Pathogenesis of Hepatopulmonary Syndrome?

American journal of respiratory cell and molecular biology·2026
Same author

Novel Role of Von Willebrand Factor-αvβ3 Integrin in Human Pulmonary Artery Smooth Muscle Cell.

American journal of respiratory cell and molecular biology·2026
Same author

Inactivation of the Phosphatase Activity of Soluble Epoxide Hydrolase Modulates SIRT3 and Attenuates Experimental Pulmonary Hypertension.

Comprehensive Physiology·2026

相关实验视频

Updated: May 12, 2026

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies
09:08

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

Published on: May 24, 2021

肺高血压中内皮细胞和光滑肌肉细胞之间的交叉交谈: 血清醇诱导的光滑肌肉增生症的关键作用.

Saadia Eddahibi1, Christophe Guignabert, Anne-Marie Barlier-Mur

  • 1INSERM U651, Département de Physiologie, Hôpital H. Mondor, AP-HP, Créteil, france. saadia.eddahibi@creteil.inserm.fr

Circulation
|April 12, 2006
PubMed
概括

肺内皮细胞 (P-ECs) 在肺高血压中驱动平滑肌肉细胞生长. 在异常性肺动脉高血压 (iPH) 中,P-ECs 增加了血清的产生,有助于这种增生.

更多相关视频

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
08:59

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Published on: May 5, 2022

相关实验视频

Last Updated: May 12, 2026

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies
09:08

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

Published on: May 24, 2021

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
08:59

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Published on: May 5, 2022

科学领域:

  • 血管生物学 血管生物学
  • 细胞信号传输 细胞信号传输
  • 肺高血压的病理生理学

背景情况:

  • 肺动脉光滑肌细胞 (PA-SMC) 增生是异常性肺动脉高血压 (iPH) 的标志.
  • 无论是内在的PA-SMC特征还是外部刺激,都可能有助于iPH的发病.
  • 肺微血管内皮细胞 (P-ECs) 在调节PA-SMC生长中的作用尚未完全阐明.

研究的目的:

  • 研究P-ECs在控制PA-SMC扩散中的作用.
  • 确定影响PA-SMC增长的P-EC产生的特定因素.
  • 为了确定P-EC衍生因素是否在iPH中失调.

主要方法:

  • 从IPH患者和对照组培养了静止的P-EC和PA-SMC.
  • 评估了对P-EC条件介质的反应中PA-SMC的扩散.
  • 利用素和p-甲氨酸来研究血清素的作用.
  • 测量了基酶 (TPH) 活性和tph1基因表达.

主要成果:

  • P-EC 条件介质显著增加了 PA-SMC 的扩散,而来自 iPH 的 P-EC 和 PA-SMC 的效果更大.
  • 血清激素合成抑制剂 (fluoxetine,p-chlorophenylalanine) 减少了60%的增长促进作用;内啡素受体抑制剂没有作用.
  • 在iPH P-EC和肺部中,TPH活性和tph1基因表达升高,表明血清素的产生增加.

结论:

  • P-ECs产生了调节PA-SMC生长的副因子.
  • 血清素是一种关键的P-EC衍生生长因子,与PA-SMC增生有关.
  • 通过P-ECs产生的血清的过度产生有助于iPH的发病.