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在NO结合的细胞染色体c'的Endor
Oleg M Usov1, Peter S-T Choi, James P Shapleigh
1Department of Chemistry, Center for Biochemistry and Biophysics, University at Albany, SUNY, Albany, New York 12222, USA.
电子核双共振 (ENDOR) 揭示了细胞染色体c'中五坐标NO结合的血的详细电子结构. 这项研究精确地绘制了未配对的电子密度和血方向,提供了对联体相互作用的见解.
科学领域:
- 生物化学 生物化学
- 生物物理学的生物物理.
- 频谱学是一种光谱学.
背景情况:
- 细胞染色体c'是一种参与脱过程的血红蛋白.
- 氧化 (NO) 是一种信号分子,也是血红蛋白的潜在连接体.
- 了解NO结合的电子和结构性质对于阐明酶机制至关重要.
研究的目的:
- 为了研究来自R. sphaeroides的细胞染色体c'中的五坐标NO结合的血的电子结构.
- 为了确定未配对电子密度在氧化联体上的分布.
- 为了阐明相对于FeNO平面的海姆群的方向.
主要方法:
- 使用质子,和原子核的Q波段电子核双共振 (ENDOR) 光谱.
- 对超细合常数和二极合的分析.
- 使用氨酸模型分配ENDOR频率.
主要成果:
- 的高精度合范围为14NO的36-44 MHz和15NO的51-63 MHz.
- 在NO上的旋转密度分布在具有2s和2p字符 (1:2比) 的轨道中.
- FeNO平面切割了血红素N-Fe-N角,Phe14和Arg127残留物分别位于血红素铁和NO连接体附近.
结论:
- ENDOR是一种强大的技术,用于探测NO结合的血红蛋白中的电子自旋密度和血红蛋白方向.
- 联体的电子结构表明,复杂的结合相互作用超出了简单的pi轨道.
- 结构分析表明Phe14和Arg127在调节NO结合和功能的潜在作用.
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