Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A nature-inspired ion trap for parallel manipulation of ions on a massive scale.

Science advances·2026
Same author

Plasma proteomic profiling reveals distinct protein signatures associated with hepatocellular carcinoma in chronic hepatitis B infection.

Clinical proteomics·2026
Same author

Division of labor in trypanosome RNA processing and export through expanded Mex67 paralogs.

Nucleic acids research·2026
Same author

Karyopherins remodel the dynamic organization of the nuclear pore complex transport barrier.

Nature cell biology·2025
Same author

Distinct quaternary states, intermediates, and autoinhibition during loading of the DnaB-replicative helicase by the phage λP helicase loader.

Nucleic acids research·2025
Same author

Evaluation of the Activity of Monensin and Its Analogs for Modulation of Stem-like Cell Functionality in 2D and 3D Breast Cancer Models.

ACS pharmacology & translational science·2025

相关实验视频

Updated: Jun 19, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
13:20

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Published on: July 30, 2010

在生产性激活剂依赖的染色质模板转录中,SII和p300的协同功能.

Mohamed Guermah1, Vikas B Palhan, Alan J Tackett

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. guermam@mail.rockefeller.edu

Cell
|April 25, 2006
PubMed
概括

研究人员确定了一种染色体转录激活活性 (CTEA),该活性可以通过核体促进RNA聚合酶II的延长. 这种活动涉及p300和转录因子SII的基因乙化,对于染色质模板上的基因表达至关重要.

更多相关视频

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

相关实验视频

Last Updated: Jun 19, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
13:20

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Published on: July 30, 2010

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 染色体生物学 染色体生物学

背景情况:

  • 在染色质模板上的RNA聚合酶II (Pol II) 转录是复杂和受调的.
  • 已知p300的素乙化促进了转录.
  • 通过核细胞体进行有效的转录延长对于体内基因表达是必不可少的.

研究的目的:

  • 重建一个无细胞系统,用于研究染色体上的转录.
  • 识别能够在染色质上实现生产性转录延长的因素.
  • 阐明基因素乙化和特定转录因子在染色体转录中的作用.

主要方法:

  • 用纯化的组件重建一个体外转录系统.
  • 使用纯化基因素和DNA组装染色素模板.
  • 染色体转录激活活性 (CTEA) 的净化和表征.
  • 在DNA和染色质模板上进行转录启动和延长的测试.

主要成果:

  • 重建后的系统支持激活剂依赖的启动,但不能在染色质上产生延伸.
  • 净化了一种新的染色体转录激活活性 (CTEA).
  • CTEA以p300和乙-CoA依赖的方式通过核细胞增强了转录延长.
  • 转录延长因子SII是CTEA的关键组成部分,与p300协同作用.
  • 鉴定出HMGB2是一种增强SII和p300功能的联合激活剂.

结论:

  • 在染色质上产生转录延长需要超出一般转录机制的特定因素.
  • 含有SII且受p300介导的组素乙化影响的CTEA对于克服核体障碍至关重要.
  • HMGB2作为一种协活性剂,调节SII和p300在染色体转录中的功能.