相关实验视频
Updated: Jul 15, 2026

16:19
High Throughput Single-cell and Multiple-cell Micro-encapsulation
Published on: June 15, 2012
控制细胞,蛋白质和微粒在脂质囊中的微流体封装
Yung-Chieh Tan1, Kanaka Hettiarachchi, Maria Siu
1University of California Irvine, Department of Biomedical Engineering, Irvine, California 92697, USA. ytan@uci.edu
Journal of the American Chemical Society
|April 28, 2006
概括
一种新的微流体技术快速制备脂质囊泡,用于细胞封装,无需有毒溶剂. 这种方法为细胞生物学和药物输送中的应用提供了受控的封装效率和一致的囊泡大小.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 细胞生物学 细胞生物学
背景情况:
- 脂质囊泡对于封装生物材料至关重要.
- 传统方法通常涉及有毒溶剂,缺乏精确的控制.
- 开发高效且无毒的囊泡配方技术至关重要.
研究的目的:
- 引入一种新的微流体技术,用于快速的脂质囊泡配方.
- 为了证明各种有效载荷的封装,包括蛋白质,细胞和珠子.
- 使用微流体参数控制封装效率和囊泡大小.
主要方法:
- 使用微流体装置进行脂质囊泡制剂.
- 在囊泡合成中采用无溶剂的方法.
- 通过调整微流体流速来控制封装.
- 对于不同的有效载荷,具有特征的囊泡大小和封装效率.
主要成果:
- 脂质囊泡是在几分钟内形成的.
- 实现了绿色光蛋白 (GFP),癌细胞和珠子的封装.
- 囊泡的平均直径为27.2微米 (GFP),62.4微米 (细胞) 和55.9微米 (珠).
- 囊泡大小的变化为GFP和细胞约20%,珠子约10%.
结论:
- 微流体技术为脂质囊泡配方提供了一种快速,高效和无毒的方法.
- 这种方法可以控制各种生物和非生物材料的封装.
- 该方法显示了在细胞封装和药物递送系统中的应用潜力.
相关概念视频
Eukaryotic Compartmentalizations
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...
Membrane Fluidity
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Membrane Fluidity
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Membrane Domains
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Assembly of the Lipid Bilayer in the ER
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Eukaryotic Compartmentalization
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...

