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工程四倍折叠:核酸构造决定了瓜四倍折叠中的折叠拓和分子性

Chung-Fei Tang¹, Richard H Shafer

¹Department of Pharmaceutical Chemistry, School of Pharmacy and Graduate Group in Chemistry and Chemical Biology, University of California, San Francisco, California 94143-0446, USA.

Journal of the American Chemical Society

|April 28, 2006

概括

核酸四重复折叠拓是由关氨酸核酸体构成控制的. 替代DNA脱氧氨酸 (dG) 的RNA氨酸 (rG) 可以逆转折叠并改变分子性.

科学领域:

结构生物学是结构生物学.
核酸化学的核酸化学
生物化学生物化学

背景情况:

核酸四重复是由富含关氨酸的序列形成的.
四个复合体折叠成平行或反平行拓.
了解控制四重折叠的因素对于生物应用至关重要.

研究的目的:

为了研究核酸体构成在确定四重复折叠拓学中的作用.
探索选择性核酸替代如何影响四重复结构和分子性.

主要方法:

在DNA和RNA体中,选择性地用核糖核酸瓜诺辛 (rG) 替代脱氧瓜诺辛 (dG).
分析四重复折叠拓和分子性的结果变化.

主要成果:

将rG替换为dG,反转了血栓结合性阿巴胺 (TBA) 的反平行折叠.
这种替代将单分子四重体转换为双分子四重体.
在一个全RNA TBA 模拟中,类似的替代将拓从平行转移到反平行,并改变了链状结构.

结论:

瓜宁核糖核酸对抗形状的强烈偏好推动了四重复拓学的变化.
关氨酸核酸体构造决定了四重折叠,分子性,并提供了结构控制的方法.

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