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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

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相关实验视频

Updated: Jul 24, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

在人类老龄化过程中,依赖于层A的核缺陷.

Paola Scaffidi1, Tom Misteli

  • 1National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.

Science (New York, N.Y.)
|April 29, 2006
PubMed
概括

导致Hutchinson-Gilford发病症 (HGPS) 的相同拼接缺陷也发生在健康的衰老细胞中. 抑制这种层A拼接部位可以逆转与年龄相关的核缺陷,使层A在生理衰老中产生影响.

科学领域:

  • 细胞生物学 细胞生物学
  • 遗传学 是一个遗传学.
  • 衰老研究研究 衰老研究

背景情况:

  • 层状A蛋白中的突变会导致哈森-吉尔福德发育症 (HGPS),这是一个过早衰老综合征.
  • 层A在正常生理衰老中的作用尚不清楚.

研究的目的:

  • 为了研究层A在健康个体的衰老过程中的参与.
  • 为了确定HGPS背后的分子机制在正常衰老中是否活跃.

主要方法:

  • 从老年个体的细胞中分析核结构蛋白层A.
  • 在老化的细胞中评估基因素修饰和DNA损伤.
  • 调查A层中的神秘拼接部位激活及其抑制.

主要成果:

  • 来自老年人的细胞核表现出类似于HGPS细胞的缺陷,包括改变的基因组修饰和增加的DNA损伤.
  • 在健康的衰老细胞中观察到A层中密码拼接部位的零星激活,与引起HGPS的同样.
  • 抑制这种特定的层层A拼接部位成功地逆转了与年龄相关的核缺陷.

结论:

  • 驱动HGPS的分子机制在健康的衰老中起作用.

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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

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Last Updated: Jul 24, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
08:53

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

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  • 层状A层在生理衰老中起着重要作用.
  • 准层状A拼接部位可能为与年龄相关的核功能障碍提供治疗潜力.