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相关概念视频

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Transduction01:16

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

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相关实验视频

Updated: May 21, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

对于Wnt配体的分泌,需要Evi,一种保存的跨膜蛋白质.

Kerstin Bartscherer1, Nadège Pelte, Dierk Ingelfinger

  • 1German Cancer Research Center, Boveri-Group Signaling and Functional Genomics, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

Cell
|May 9, 2006
PubMed
概括
此摘要是机器生成的。

连续性中断 (Evi/Wls) 是一种对Wnt分泌至关重要的跨膜蛋白. 它的耗尽破坏了Wnt信号传递,突出了它在释放这些必不可少的信号分子中的作用.

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Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
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Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients

Published on: February 11, 2017

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

相关实验视频

Last Updated: May 21, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
07:04

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients

Published on: February 11, 2017

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

科学领域:

  • 细胞生物学 细胞生物学
  • 发育生物学是发展生物学.
  • 分子遗传学 分子遗传学

背景情况:

  • Wnt信号通路调节发育和疾病中的关键生物过程.
  • Wnt蛋白质是分泌的信号分子,在短距离和长距离之间起作用.
  • Wnt蛋白释放和分泌因子的机制在很大程度上是未知的.

研究的目的:

  • 确定涉及无翼 (Wg) 的分泌的跨膜蛋白质,这是Wnt.的Drosophila同类物.
  • 阐明Evenness中断 (Evi/Wls) 在Wnt分泌和信号传递中的作用.

主要方法:

  • 在Drosophila Wg信号传递中检测跨膜蛋白的RNA干扰 (RNAi) 选.
  • 对进基突变物发育模式缺陷的分析.
  • 经验实验和克隆分析.
  • 在人类细胞中耗尽人类Wls同类物来评估保存的功能.

主要成果:

  • 鉴定了一种保存的多通道跨膜蛋白,即Evenness中断 (Evi/Wls).
  • 艾维突变体表现出发育模式缺陷和失败的Wg目标基因表达.
  • 人类Wls的枯竭破坏了人体细胞中的Wnt信号,证实了进化保护.
  • Evi/Wls 在产生Wg的细胞内起作用,在突变细胞中保留Wg.

结论:

  • Evi/Wls对于Wg/Wnt蛋白质的分泌是必不可少的.
  • Evi/Wls是第一个被确定为Wnt分泌所需的基因家族的成员.
  • 了解Evi/Wls的功能,可以了解Wnt分泌机制,这些机制对发育和疾病至关重要.