Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Implementation of safety management systems (SMS) in healthcare: a systematic review and international comparison.

BMJ open·2026
Same author

Targeted Sensitization of Leukemic T-cells to Anticancer Drugs by SIRT1 Agonist SRT-1720.

Anticancer research·2026
Same author

Multi-spin Redox Sensor for Electron Paramagnetic Resonance Studies of Tissue Redox State <i>In Vivo</i>: Validation of Data With a Conventional Spin Probe.

In vivo (Athens, Greece)·2025
Same author

Amplitude-integrated electroencephalography compared with conventional video-electroencephalography for detection of neonatal seizures.

The Cochrane database of systematic reviews·2025
Same author

An Electron Paramagnetic Resonance Study of the Superoxide-Scavenging and Redox-Modulating Effects of Lecithinized Superoxide Dismutase in the Bloodstream.

Molecules (Basel, Switzerland)·2025
Same author

Changes in the Erythrogram Parameters and in the Erythrocyte Sizes of Adult <i>Pelophylax ridibundus</i> (Pallas 1771) (Anura: Ranidae) Inhabiting the Sedimentation Lake of Brikel Thermal Power Plant in Southern Bulgaria.

Toxics·2025

相关实验视频

Updated: Jul 10, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

单个量子点微粒被皮覆盖,作为潜在的非细胞毒性光细胞标记剂.

Zhivko Zhelev1, Hideki Ohba, Rumiana Bakalova

  • 1On-Site Sensing and Diagnosis Research Laboratory, National Institute of Advanced Industrial Science and Technology, AIST-Kyushu, 807-1 Shuku-machi, Tosu 841-0052, Japan.

Journal of the American Chemical Society
|May 11, 2006
PubMed
概括
此摘要是机器生成的。

研究人员在二氧化外内稳定了单个量子点 (QD),增强了它们的光发光,并使细胞追踪和药物输送等应用成为可能. 这些生物相容的纳米粒子提供了可控的大小,并防止聚合.

更多相关视频

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling
07:51

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

Published on: March 20, 2026

相关实验视频

Last Updated: Jul 10, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling
07:51

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

Published on: March 20, 2026

科学领域:

  • 材料科学 材料科学 材料科学
  • 纳米技术 纳米技术
  • 生物医学工程 生物医学工程

背景情况:

  • 量子点 (QD) 具有独特的光学特性,但在水溶液中经常受到聚合和不稳定的影响.
  • 稳定QD对于其在生物和材料科学领域的实际应用至关重要.
  • 开发强大的封装方法是保持QD功能和启用新应用的关键.

研究的目的:

  • 开发一种使用前体稳定单个量子点 (QD) 的方法.
  • 在水溶液中创建具有增强光发光特性的状QD微粒.
  • 评估这些纳米粒子在生物医学应用中的潜力,包括细胞追踪和药物输送.

主要方法:

  • 使用疏水性 precursors 在小粒体内稳定单个QDs.
  • 在QD-micelle结构周围形成一个可控制厚度 (3-4 nm) 的贝.
  • 由此产生的纳米粒子的光发光特性,大小,聚合,透明度和生物相容性的表征.

主要成果:

  • 在具有外的粒中达到高达92%的单个纳米晶体加载.
  • 证明光发光强度随着外厚度的增加而增加.
  • 外的QD微粒呈现出高量子产量,可控制的小尺寸,清晰的辐射光谱,没有聚合,在水溶液中具有高透明度.
  • 在QDs附近的疏水分子的融入是由中间疏水层促进的.
  • 纳米粒子是氨基功能化的,具有良好的生物相容性,并且在长期细胞化过程中表现出非细胞毒性.

结论:

  • 外的QD微粒为增强水性环境中的QD光发光提供了一个稳定而坚固的平台.
  • 开发的纳米粒子适用于各种应用,包括细胞内输送,细胞追踪和药物输送,因为它们的生物相容性和功能化.
  • 混合材料结合了QD属性的好处,以及外提供的小尺寸和稳定性的优势.