聚A) 聚合酶和一个可分离的多基化刺激因子,其编码由疫苗病毒
P D Gershon1, B Y Ahn, M Garfield
1Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Cell
|September 20, 1991
概括
研究人员确定了两种疫苗病毒蛋白质,VP55和VP39,对于添加mRNA的多元A尾巴至关重要. VP55起到酶的作用,而VP39结合聚A并刺激尾巴的形成,揭示了mRNA处理的新机制.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因表达 基因表达
背景情况:
- 细胞mRNA通常具有3'多A尾巴,该尾巴在转录后被添加.
- 3'多A) 尾部形成的精确机制尚未完全理解.
研究的目的:
- 为了研究3' poly (A) 尾巴形成的机制.
- 为了识别和表征病毒蛋白质,负责在疫苗病毒中的多基化.
主要方法:
- 鉴定和净化疫苗病毒多VP55和VP39.
- 使用纯化的蛋白质和抗体对多基化活性进行检测.
- 分析蛋白质相互作用和多A结合性质.
主要成果:
- 原料依赖的多化活性仅与VP55-VP39异构分子有关.
- 多聚A) 聚合酶活性存在于VP55,而VP39是长聚A分子合成和自由聚A结合所需的.
- 在序列数据库中没有发现VP55或VP39的同类.
结论:
- 聚A尾部的形成包括一种催化聚 (VP55) 和一种可解离的聚A结合刺激因子 (VP39).
- 这些病毒蛋白代表了一种新型的多基化因子类,与已知的原生生物或真核生物酶不同.
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