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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

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相关实验视频

Updated: Jun 25, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

一种系统方法来绘制DNA损伤反应途径的地图.

Christopher T Workman1, H Craig Mak, Scott McCuine

  • 1University of California San Diego, La Jolla, CA 92093, USA.

Science (New York, N.Y.)
|May 20, 2006
PubMed
概括
此摘要是机器生成的。

细胞未能对DNA损伤做出反应,可能导致突变. 这项研究绘制了控制酵母这种反应的转录网络,揭示了参与DNA损伤信号的关键相互作用和途径.

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Published on: August 21, 2021

相关实验视频

Last Updated: Jun 25, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 系统生物学 系统生物学

背景情况:

  • 细胞对DNA损伤的反应对于预防突变发生和减轻环境毒性至关重要.
  • 了解控制DNA损伤反应的转录网络对于理解细胞防御机制至关重要.

研究的目的:

  • 为绘制基因组范围的转录网络,控制酵母中的DNA损伤反应.
  • 为了确定转录因子 (TF) - 标相互作用和参与对甲基甲硫酸盐 (MMS) 反应的结合动机.
  • 构建因果途径模型,整合DNA损伤后的信号,转录和表型.

主要方法:

  • 在暴露于甲基甲硫酸盐 (MMS) 的酵母中测量了30种与损伤相关的转录因子 (TF) 的全基因组结合位置.
  • 确定了TF-标相互作用,并通过评估野生类型与TF-缺乏酵母的标基因表达变化来进行功能验证.
  • 用验证的相互作用来构建因果途径模型.

主要成果:

  • 总共有5272个TF-目标相互作用被确定,显示MMS暴露后促进体结合模式的广泛变化.
  • 发现了损伤特定的结合动机,为DNA损伤反应的调节机制提供了洞察力.
  • 功能验证证实了许多对MMS的转录反应至关重要的TF-目标相互作用.

结论:

  • 这项研究揭示了一个复杂的转录网络,它控制了酵母菌的DNA损伤反应.
  • 鉴定到的TF-目标相互作用和途径为细胞如何在DNA损伤后整合信号传递,转录和表型结果提供了整体视图.
  • 这为了解分子层面的突变发生和环境毒性提供了基础.