一种质细胞骨蛋白调节蛋白质合成和上皮细胞生长
Seyun Kim1, Pauline Wong, Pierre A Coulombe
1Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Nature
|May 20, 2006
概括
蛋白17是一种中间丝蛋白,通过通过Akt/mTOR通路影响蛋白质合成来调节细胞生长. 这一发现突出了细胞骨.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞生长,一个基本的过程,受到严格的调节.
- 拉巴amycin (mTOR) 信号通路的Akt/哺乳动物点对于控制蛋白质合成和生物体生长至关重要.
- 快速细胞生长对于诸如组织修复之类的生理过程至关重要.
研究的目的:
- 为了研究质素17在调节细胞生长中的作用.
- 阐明质素17影响蛋白质合成和细胞大小的机制.
- 探索质素17,14-3-3西格玛和Akt/mTOR通路之间的联系.
主要方法:
- 在受伤的分层表皮质中分析素17表达.
- 对小鼠皮肤缺乏角质素的角质细胞的研究 17.
- 评估蛋白质翻译,细胞大小和Akt/mTOR信号活动.
- 在质素17中特定的氨基酸残留物的识别,涉及14-3-3西格玛重新定位和mTOR激活.
主要成果:
- 在组织修复过程中诱导的氨酸17,通过与14-3-3sigma结合来调节细胞生长.
- 缺乏氨酸17的氨酸细胞表现出减少的蛋白质翻译和更小的尺寸,与减少的Akt/mTOR信号相关.
- 素17中的特定残留物调解14-3-3西格玛核至细胞质转移,刺激mTOR活动和细胞生长.
结论:
- 氨酸17在调节细胞生长和大小方面发挥着新的作用.
- 中间线丝细胞骨架影响蛋白质合成和细胞生长.
- 素17通过14-3-3sigma作用,调节Akt/mTOR通路,影响细胞生长.
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