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相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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相关实验视频

Updated: Jul 10, 2026

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

在同卵性小鼠中,微妙的小脑表型用于针对性地删除En-2 homeobox.

A L Joyner1, K Herrup, B A Auerbach

  • 1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.

Science (New York, N.Y.)
|March 8, 1991
PubMed
概括

鼠标En-1和En-2基因,Drosophila engrailed的同类物,参与神经发育. 虽然En-2突变小鼠由于冗余性而没有表现出胚胎缺陷,但成年大脑小叶异常,突出显示了En-2的特定作用.

科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 神经科学是一个神经科学.

背景情况:

  • 嵌入的Drosophila细分基因在小鼠中具有同类,En-1和En-2.
  • 这些基因在胚胎神经管中表现出重叠的表达,这表明它们在区域特征中的作用.
  • En-1和En-2是Drosophila细分基因的同类物.

研究的目的:

  • 研究En-1和En-2基因在小鼠胚胎发生和成人的发育过程中的功能作用.
  • 确定En-1和En-2之间是否存在功能冗余.
  • 阐明En-2对小脑发育的特殊贡献.

主要方法:

  • 产生具有向突变并删除En-2中的homeobox的小鼠.
  • 在同卵性突变小鼠中观察胚胎发育.
  • 在突变小鼠中分析成年小脑结构,特别是叶片.

主要成果:

  • 对于En-2突变同卵性小鼠是可行的,并且在胚胎发育过程中没有明显的缺陷.
  • 缺乏胚胎缺陷表明,在胚胎发生过程中,En-1和En-2之间存在功能冗余.
  • 突变小鼠在成年小脑中表现出异常的叶子,这是一个通常仅表达En-2的区域.

更多相关视频

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
07:43

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Published on: May 12, 2015

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

相关实验视频

Last Updated: Jul 10, 2026

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
07:43

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Published on: May 12, 2015

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

结论:

  • 在En-1和En-2之间的功能冗余掩盖了潜在的胚胎缺陷,当En-2发生突变时.
  • 在成年小脑的正确叶状形成中,En-2起着关键的,非冗余的作用.
  • 这些发现凸显了同源基因在复杂生物体中具有独特的发育作用.