在HIV-1 syncytium抑制抗体和儿童的临床结果之间的关系
T J Brenner1, K E Dahl, B Olson
1Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510.
Lancet (London, England)
|April 27, 1991
概括
合成体抑制 (SI) 抗体有助于防止HIV-1细胞间传播. 这些抗体在大多数HIV-1阳性儿童中被发现,特别是那些患有淋巴细胞间歇性肺炎的儿童,与临床结果相关.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 儿科 儿科 儿科
背景情况:
- 同胞体的形成是HIV-1细胞间传播和细胞病理学的关键机制.
- 抑制同位素形成的抗体 (SI抗体) 可能在控制病毒传播方面发挥作用.
研究的目的:
- 开发和验证一种syncytium抑制 (SI) 试验,用于测量针对HIV-1诱导的syncytia的抗体.
- 调查艾滋病毒-1 感染母亲产生的儿童中SI 抗体的存在和临床意义.
主要方法:
- 开发一种使用CD4+标志细胞和HIV-1感染的融合细胞来诱导合成细胞形成的共同培养试验.
- 测试了34名儿童的血清,使用西斑 (Western blot,简称WB) 和SI测试.
- 与淋巴细胞间歇性肺炎 (LIP) 和机会性感染 (OI) 等临床疾病的SI抗体患病率和标位的相关性分析.
主要成果:
- 在65%的WB阳性儿童中检测到SI抗体,但没有WB阴性儿童.
- 与OI (28.6%,标位20) 相比,患有LIP的儿童表现出高患病率 (100%) 和较高的SI抗体标位 (40-320+).
- 在血清逆转的儿童中,SI抗体不存在,它们的存在与临床结果有显著的相关性 (p < 0.05).
结论:
- SI测定是一种有价值的工具,用于检测抑制HIV-1介导合成形成的抗体.
- 艾滋病毒-1感染儿童中存在SI抗体,与特定的临床表现相关,特别是LIP.
- 识别负责合成体形成的HIV-1表位对了解SI抗体在疾病进展中的作用以及潜在的治疗策略至关重要.
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