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相关概念视频

The Carbon Cycle01:14

The Carbon Cycle

Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle.
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
Microbes and the Carbon Cycle01:24

Microbes and the Carbon Cycle

The carbon cycle is a fundamental Earth process involving the transfer of carbon among the biosphere, lithosphere, atmosphere, and hydrosphere. It plays a critical role in regulating the planet’s climate and supporting life by cycling carbon through various chemical forms and reservoirs. Carbon primarily circulates as carbon dioxide (CO₂), representing its oxidized form, while reduced forms such as methane (CH₄) and organic compounds also play essential roles.Microbial activity is central to...
Microbes and Methanogenesis01:26

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...
Microbes and the Sulfur Cycle01:29

Microbes and the Sulfur Cycle

Sulfur is a vital element in Earth's biogeochemical systems. It transitions through various inorganic states, including sulfate (SO₄²⁻), elemental sulfur (S⁰), and sulfide (S²⁻). Abiotic and biological mechanisms across oxic and anoxic environments intricately mediate these transformations. Sulfate, the most oxidized form of sulfur, is predominantly stored in rocks, marine sediments, and oceanic waters, acting as a long-term reservoir in the global sulfur cycle.In oxic environments,...

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相关实验视频

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Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

在浮游生物微藻中通过氧酶/氧氧化物酸酶介导的转化形成化中链碳化合物.

Thomas Wichard1, Georg Pohnert

  • 1Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Journal of the American Chemical Society
|June 1, 2006
PubMed
概括

像Stephanopyxis turris这样的海洋藻迅速转化为eicosapentaenoic酸. 这项研究揭示了一种新的酶途径,产生化碳化合物和不和氧酸.

科学领域:

  • 海洋生物学 海洋生物学
  • 生物化学 生物化学
  • 酶学 是一种酶学.

背景情况:

  • 海洋生物中的乙酸代谢尚未完全理解.
  • 已知的脂肪酸裂变活动并不解释观察到的产品.

研究的目的:

  • 为了阐明海洋藻Stephanopyxis turris中eicosapentaenoic酸转化的生化途径.
  • 确定参与生产化碳化合物和不和氧酸的酶.

主要方法:

  • 使用标记的前体来追踪代谢途径.
  • 陷实验用于分离中间产品.
  • 选择性抑制酶活动以确定特定的作用.

主要成果:

  • 斯蒂法诺皮克西斯 (Stephanopyxis turris) 细胞的分解迅速转化为乙酸.
  • 一个新的途径涉及氧酶激活脂肪酸到氧化物.
  • 一个新的氧化醇酶将氧化分解成化C8碳化合物和不和C12氧酸.

结论:

  • 在大自然中发现了一种新的化酶途径.
  • 这一途径有助于海洋藻的独特生物化学.

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  • 这些发现扩大了已知的酶类素化活动的范围.