半孔二氧化-磁铁纳米复合材料:在磁性生物分离中的制造和应用
Tapas Sen1, Antonio Sebastianelli, Ian James Bruce
1Department of Biosciences, University of Kent at Canterbury, Canterbury, Kent, CT2 7NJ, United Kingdom. t.sen@kent.ac.uk
Journal of the American Chemical Society
|June 1, 2006
概括
使用新型纳米复合材料的磁性生物分离有效捕获和释放核酸. 这一进步为核酸净化和分析提供了一种新方法.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 纳米技术纳米技术
背景情况:
- 核酸净化对于分子生物学和诊断至关重要.
- 现有的方法可能耗时或需要昂贵的试剂.
- 超偏磁纳米材料提供了有效生物分离的潜力.
研究的目的:
- 开发和描述一个半孔性超对磁性-磁铁纳米复合材料.
- 为了评估这个纳米复合物的核酸磁性生物分离的效率.
- 为了证明核酸回收的吸附和化能力.
主要方法:
- 一个半孔的二氧化-磁铁的纳米复合物的合成.
- 纳米复合材料的结构和磁性特性的表征.
- 使用核酸的吸附-化试验进行性能评估.
主要成果:
- 合成的纳米复合材料表现出超对磁性质.
- 实现了核酸对纳米复合材料的有效吸附.
- 通过化证明了纯化的核酸的高回收率.
结论:
- 半孔性超偏磁性-磁铁纳米复合物对核酸的磁性生物分离是有效的.
- 这种材料为核酸净化提供了一个有前途的平台.
- 开发的方法为核酸回收提供了一个简单,快速和高效的方法.
相关概念视频
Applications Of NMR In Biology
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
The...
Magnetism
Magnets are commonly found in everyday objects, such as toys, hangers, elevators, doorbells, and computer devices. Experimentation on these magnets shows that all magnets have two poles: one is labeled north (N) and the other south (S). Magnetic poles repel if they are alike and attract if unlike. Moreover, both poles of a magnet attract unmagnetized pieces of iron.
An individual magnetic pole cannot be isolated. No matter how small, every piece of a magnet contains a north pole and a south...
An individual magnetic pole cannot be isolated. No matter how small, every piece of a magnet contains a north pole and a south...
Ferromagnetism
Materials like iron, nickel, and cobalt consist of magnetic domains, within which the magnetic dipoles are arranged parallel to each other. The magnetic dipoles are rigidly aligned in the same direction within a domain by quantum mechanical coupling among the atoms. This coupling is so strong that even thermal agitation at room temperature cannot break it. The result is that each domain has a net dipole moment. However, some materials have weaker coupling, and are ferromagnetic at lower...
Magnetic Susceptibility and Permeability
In linear magnetic materials, like paramagnets and diamagnets, magnetization is proportional to the magnetic field intensity. The constant of proportionality, a dimensionless number, is called magnetic susceptibility. The value of the susceptibility depends on the type of material.
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
Other Unique Bacteria
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic and are commonly found near the...


