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通过单分子成像和光共振能量转移研究的MjHsp16.5子单位交换

Yinghua Guan1, Zheng Wang, Aoneng Cao

  • 1Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Journal of the American Chemical Society
|June 1, 2006
PubMed
概括

单分子成像揭示了MjHsp16.5.5.中的温度依赖子单位交换. 在75°C以下,完整的二分体解离极限交换;在75°C以上,形成较小的单元,表明双分子反应机制.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...