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用PCR指纹来选择HLA匹配的不相关的骨髓捐赠者. 在IMUST研究中的合作中心

T M Clay¹, J L Bidwell, M R Howard

¹UK Transplant Service, Bristol.

Lancet (London, England)

|May 4, 1991

概括

一种新的聚合酶连锁反应 (PCR) 指纹检测技术可以快速识别与人无关的骨髓捐赠者的人类白细胞抗原 (HLA) -DR不匹配. 这种方法可以加快供体选择,提高患者的存活率.

科学领域:

免疫遗传学免疫遗传学
分子生物学分子生物学
血液学血液学血液学

背景情况:

人类白细胞抗原 (HLA) 类型对非相关的骨髓捐赠者选择至关重要.
HLA匹配的延迟有助于患者的生存率低下.
当前的HLA类型化方法可能会耗时.

研究的目的:

评估一种新的DNA技术,用于患者和非相关的骨髓捐赠者之间的HLA-DR匹配.
评估PCR指纹的潜力,以加速供体选择.

主要方法:

该技术利用聚合酶连锁反应 (PCR) 期间的异重复形成来产生独特的带纹图案 (PCR指纹).
通过视觉比较患者和捐赠者的指纹来进行HLA-DR匹配.
在最后的PCR阶段之前,DNA交叉匹配通过混合DNA来确认身份.

主要成果:

在53个患者-捐赠者对中的42个中,PCR指纹检测结果与DNA-RFLP分析一致.
通过PCR指纹检测发现了标准DNA-RFLP遗漏的11对DR/Dw不匹配.
序列特定的寡核酸 (SSO) 类型确定DR4亚型是不匹配的原因.

结论:

PCR指纹检测是HLA-DR匹配在无关骨髓捐赠者搜索中的可行方法.
这种技术有可能通过简化后勤来加速供体选择.
更快的供体选择可能会导致改善患者的生存结果.

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