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相关概念视频

DNA Packaging00:58

DNA Packaging

Overview
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
DNA Packaging00:58

DNA Packaging

Overview
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

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相关实验视频

Updated: Jul 9, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

基因组中的TFIIIC盒子

Lori L Wallrath1, Pamela K Geyer

  • 1Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.

Cell
|June 6, 2006
PubMed
概括

乙盒和TFIIIC蛋白质限制了酵母中异染色素的传播. TFIIIC结合点聚集在核边缘,表明该复合体具有全基因组的屏障功能.

科学领域:

  • 遗传学和分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 酵母基因组学 酵母基因组学

背景情况:

  • 对基因组稳定性和基因调节而言,异色染色体的形成至关重要.
  • 限制异性染色素传播的机制尚未完全理解.
  • 分裂酵母作为研究色素动态的模型生物.

研究的目的:

  • 调查B盒和TFIIIC在裂变酵母中的异染色素调节中的作用.
  • 为了确定TFIIIC的基因组分布和功能.
  • 为了确定限制异性染色素传播的因素.

主要方法:

  • 染色体免疫沉 (ChIP) 用于TFIIIC局部化.
  • 在特定的基因组位置分析异性染色素标记物.
  • 对TFIIIC结合位点的全基因组分析.

主要成果:

  • 发现B盒和TFIIIC可以限制异染色素在无声交配类型区域的传播.
  • 全球分析显示,TFIIIC的协会网站分散在各个地方.
  • TFIIIC结合点最好聚集在核周边地区.

结论:

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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  • TFIIIC在防止异性染色素的不受控制的传播方面发挥着重要作用.
  • TFIIIC可能充当基因组屏障,维持不同的染色质状态.
  • TFIIIC在核外围的定位表明它在组织基因组架构中的作用.