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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Regulation of Angiogenesis and Blood Supply01:24

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Catenins01:23

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Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
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Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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相关实验视频

Updated: May 6, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
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p38和与p38相互作用的蛋白质对于在小鼠胃化过程中对E-cadherin的下调至关重要.

Irene E Zohn1, Yingqiu Li, Edward Y Skolnik

  • 1Howard Hughes Medical Institute, Department of Pediatrics, Section of Developmental Biology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA.

Cell
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概括
此摘要是机器生成的。

p38 MAP 激酶和p38 相互作用蛋白 (p38IP) 通过降低 E-cadherin 的调节,对于脊椎动物胃流动期间的中皮层迁移至关重要. 在p38IP中的突变破坏了这个过程,导致发育缺陷.

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科学领域:

  • 发育生物学 发展生物学
  • 细胞信号传递 细胞信号传递
  • 分子遗传学 分子遗传学

背景情况:

  • 脊椎动物的胃化涉及表皮细胞到介质细胞的过渡 (EMT),用于介质体的迁移.
  • 在消化过程中,对EMT而言,E-cadherin的降低调节至关重要.
  • 在这个过程中,p38 MAP 激酶及其相互作用蛋白的作用需要进一步阐明.

研究的目的:

  • 调查p38 MAP激酶和p38相互作用蛋白 (p38IP) 在胃流动期间E-cadherin下调中的作用.
  • 确定影响p38IP功能的遗传因素及其对胚胎发育的影响.

主要方法:

  • ENU-突变发生屏幕用于识别影响p38IP的突变.
  • 对发育缺陷的突变胚胎 (p38IP,p38IP,RRK) 的分析.
  • 生物化学测试以确定p38IP与p38的相互作用及其在p38激活中的作用.
  • 研究E-cadherin蛋白水平和p38和p38IP的调节.

主要成果:

  • 垂落眼 (干燥) 突变影响p38IP拼接,导致发育缺陷.
  • 较强的p38IP突变会导致胃化缺陷,原因是中皮迁移受损和E-cadherin下调.
  • p38IP直接与p38结合,并且是活体中p38激活所必需的.
  • 在胃流动过程中,p38和p38IP都对E-cadherin下调有必要.
  • p38调节NCK相互作用激酶 (NIK) 下游的E-cadherin蛋白表达,并且独立于Fgf信号和Snail.

结论:

  • p38 MAP 激酶和p38IP是脊椎动物胃流动期间E-cadherin下调的关键调节者.
  • 在体内,p38IP充当了p38信号的支架或激活器.
  • 干扰p38/p38IP通路会导致胃化缺陷,因为会影响中皮层的迁移.