核孔协会为可诱导的酵母基因提供了最佳的表达水平
Angela Taddei1, Griet Van Houwe, Florence Hediger
1Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
Nature
|June 9, 2006
概括
核基因定位会影响基因表达. 将HXK1基因转移到核孔中,增强了其转录,证明了核位置.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 核被组织成分组,以促进特定的核功能.
- 在发芽的酵母菌中,沉默的染色质区域聚集在核外上,促进基因抑制.
- 基因转录已在核外围观察到,与核毛孔复合体关联.
研究的目的:
- 研究核定位在基因调节中的作用.
- 为了确定特定基因的转录激活是否导致其核定位的变化.
- 了解基因位点位置,核孔综合体和基因表达水平之间的关系.
主要方法:
- 研究了在芽酵母中转录的一个亚端粒基因HXK1的激活.
- 分析了不同激活途径 (非葡萄糖碳源与VP16交换激活器) 对HXK1局部化的影响.
- 研究了3'未翻译区域 (UTR) 在HXK1转移中的作用.
- 操纵了HXK1与核孔的关联,并测量了转录水平.
主要成果:
- 通过非葡萄糖碳源对HXK1的转录激活导致其重新定位到核孔.
- 这种转移取决于HXK1.1的3' UTR.
- 通过VP16交易激活器的激活使HXK1远离核外围,并损害了银河糖诱导.
- 干扰核孔中的HXK1局部化改变了转录水平,在对抗时降低水平,在促进协会时提高水平.
结论:
- 核位置在确定最佳基因表达水平方面发挥着积极作用.
- 基因的定位,特别是它们与核孔综合体的关联,是调节转录的关键因素.
- 3' UTR 在转录激活过程中对基因的调节定位很重要.
相关概念视频
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Protein Sorting
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Nuclear Localization Signals and Import
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Nuclear Export
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...


