沙佩罗宁介导的蛋白质在groEL表面通过一种类似于"化球体"的中间体折叠
Nature
|July 4, 1991
概括
通过稳定中间状态和促进有序结构的获取,Groe护理系统有助于蛋白质折叠. 这项体外研究表明,体内蛋白质折叠的一般机制.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 蛋白质折叠 蛋白质的折叠
背景情况:
- 蛋白质折叠对于细胞功能至关重要.
- 陪伴蛋白在协助蛋白质折叠中起着至关重要的作用.
- GroE护航系统 (GroEL/GroES) 参与了蛋白质折叠.
研究的目的:
- 复制和研究由GroE陪伴者系统介导的单体酶的体外折叠.
- 阐明GroEL和GroES促进蛋白质折叠的机制.
主要方法:
- 在试验室中使用纯化的GroEL和GroES复制酶折叠.
- 通过GroEL.EL对蛋白质形状稳定性的分析.
- 研究Mg-ATP和GroES在促进三级结构获得中的作用.
- 在折叠过程中量化ATP水解.
主要成果:
- GroEL稳定聚在一种类似于化的球体的形状中.
- -ATP和GroES有助于在GroEL表面获得有序的三级结构.
- 由Groe介导的蛋白质折叠需要显著的ATP水解 (大约. 100 个ATP/单体).
结论:
- 在Groe的陪伴者系统积极调解蛋白质折叠.
- 在GroEL上通过表面介导的链条折叠可能代表蛋白质结构形成的一般体内机制.
更多相关视频
相关概念视频
Protein Organization
Overview
Protein Folding
Overview
Protein Folding Quality Check in the RER
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Bacterial Protein Maturation
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...


