蛋白质吸附在有型有机薄膜阵列上的定量成像,使用二次电子发射
Nathan H Mack1, Rui Dong, Ralph G Nuzzo
1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Journal of the American Chemical Society
|June 15, 2006
概括
二次电子发射量化了使用有型有机膜对黄金表面的蛋白质结合. 这种技术具有高灵敏度的蛋白质吸附图像,与分子重量和覆盖率相关联的对比度.
科学领域:
- 表面科学是一门科学.
- 纳米技术纳米技术
- 生物物理学的生物物理.
背景情况:
- 二次电子 (SE) 发射是一种表面敏感的技术.
- 量化蛋白质表面相互作用对于生物传感器的发展至关重要.
- 图案有机薄膜可以精确控制表面的特性.
研究的目的:
- 开发二次电子发射作为量化和成像蛋白质结合的方法.
- 调查有型有机薄膜如何影响SE收益率.
- 为了证明SE成像对蛋白质测试的有用性.
主要方法:
- 在黄金表面上微接触印刷基醇和聚乙烯甘醇 (PEG) 接种的聚烯胺聚合物.
- 作为表面变化的函数,对二次电子 (SE) 产量的系统定量.
- 开发一种微流体测定方法,用于使用SE成像检测空间定位蛋白质.
主要成果:
- 自组装单层 (SAM) 的基醇增加了SE产量,与链条长度,表面潜力和湿相关.
- 基于聚合物厚度的PEGylated聚合物减弱的SE产量.
- SE成像显示了与蛋白质分子重量和质量覆盖率直接相关的灰度对比.
- 模型蛋白质测定成功测量了吸附异温和选择性蛋白质识别.
结论:
- 吸附剂的分子结构显著介导SE产量,使定量电子成像成为可能.
- SE成像为研究蛋白质表面相互作用提供了一种灵敏且具有空间分辨率的方法.
- 这种方法为新的生物传感和表面分析应用提供了基础.
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