Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.

PLoS biology·2026
Same author

Therapeutic potential of dihydronicotinamide riboside (NRH) on obesity and glucose intolerance in mice.

Nature communications·2026
Same author

Glycolytic Specialization Shapes Neuronal Physiology and Function <i>in vivo</i>.

bioRxiv : the preprint server for biology·2026
Same author

MEDAG functions as an A-kinase-anchoring protein in adipocytes.

Molecular cell·2026
Same author

Designing synthetic regulatory elements using the generative AI framework DNA-Diffusion.

Nature genetics·2025
Same author

Inferring binding specificities of human transcription factors with the wisdom of crowds.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Jun 27, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

一个基因中心的C. elegans蛋白质-DNA相互作用网络.

Bart Deplancke1, Arnab Mukhopadhyay, Wanyuan Ao

  • 1Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA.

Cell
|June 17, 2006
PubMed
概括

这项研究绘制了C. elegans中转录因子 (TF) 相互作用的地图,使用基因中心的酵母单杂交试验. 它揭示了一个高度连接的蛋白质-DNA相互作用网络,识别了新的TF,并提供了对基因调节的见解.

更多相关视频

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 26, 2013

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

相关实验视频

Last Updated: Jun 27, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 26, 2013

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 系统生物学 系统生物学

背景情况:

  • 转录调节网络通过转录因子 (TF) 和点基因之间的相互作用来控制基因表达.
  • 与单细胞生物相比,在甲状动物系统中绘制这些相互作用是具有挑战性的.
  • 现有的以TF为中心的方法对复杂的多细胞生物来说不太适合.

研究的目的:

  • 使用基因为中心的方法,系统地绘制C. elegans中转录因子-向基因相互作用的地图.
  • 为C. elegans消化道构建一个蛋白质-DNA相互作用 (PDI) 网络.
  • 为了识别新的TF,并获得对甲动物基因调节的洞察力.

主要方法:

  • 使用高通量酵母一混合 (Y1H) 试验.
  • 采用了以基因为中心的策略,专注于基因促进剂.
  • 研究了72种C. elegans消化道基因促进剂和117种蛋白质之间的相互作用.

主要成果:

  • 确定了283个蛋白质-DNA相互作用,形成了一个高度连接的PDI网络.
  • 该网络丰富了C. elegans消化道中表达的TFs.
  • 为以前未被描述的TF提供了功能注释,并确定了十个新的假定TF.
  • 产生了支持多个PDI的体内证据.

结论:

  • 以基因为中心的方法对于剖析元动物转录调节网络具有强大作用.
  • 构建的PDI网络为系统层面的差异性基因表达提供了宝贵的见解.
  • 这项研究扩展了C. elegans TFs的功能注释,并揭示了新的监管组件.