Search research articles

Search research articles

相关实验视频

Updated: Jul 16, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Methods to Identify the NMR Resonances of the ¹³C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

使用[5-13C]lysine确定氨酸pK值:应用于DNAPolββ的酶域.

Guanghua Gao¹, Rajendra Prasad, Siegfried N Lodwig

¹Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.

Journal of the American Chemical Society

|June 22, 2006

概括

这项研究引入了一种使用[5-13C]lysine的新方法,以准确确定蛋白质残留的质子化状态. 这种方法克服了传统方法的局限性,使我们能够更好地了解蛋白质化学和稳定性.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Comparison of different heparin locking frequencies in patients with unremoved catheters after continuous renal replacement therapy in the intensive care unit: a prospective, open-label, randomized controlled trial protocol from China.

Trials·2026

Same author

Conservative oxygen therapy in extracorporeal membrane oxygenation for post-transplant refractory hypoxemia in a pediatric liver transplant recipient with very severe hepatopulmonary syndrome: A case report.

Medicine·2025

Same author

Precise Genomic Riboregulator Control of Metabolic Flux in Microbial Systems.

ACS synthetic biology·2022

Same author

The structure of Synechococcus elongatus enolase reveals key aspects of phosphoenolpyruvate binding.

Acta crystallographica. Section F, Structural biology communications·2022

Same author

A physiologically-based pharmacokinetic model of oseltamivir phosphate and its carboxylate metabolite for rats and humans.

ADMET & DMPK·2022

Same author

In vitro evidence of NLRP3 inflammasome regulation by histone demethylase LSD2 in renal cancer: a pilot study.

Molecular biology reports·2020

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

科学领域:

生物化学生物化学
结构生物学结构生物学
化学生物学化学生物学

背景情况:

确定蛋白质残留的质子化状态对于理解蛋白质的功能和稳定性至关重要.
传统方法面临的挑战是,蛋白质在极端pH值下展开,特别是具有高pK值的氨酸残留物.

研究的目的:

开发一种更可靠的方法来确定蛋白质残留的质子化状态,特别是对于氨酸残留物.
为[5-13C]lysine引入一种新的合成方法,并将其应用于酶研究.

主要方法:

使用lysine C-5的13C共振作为epsilon-amino组定位的记者,提供比C-6大5倍的转移.
为[5-13C]lysine开发一种新的合成过程.
在有限的pH范围内获得的定位数据上应用双参数匹配.

主要成果:

[5-13C]氨酸方法提供了准确的定位转移和pK值.
确定了DNA聚合酶β溶解酶域中的Lys72的pK值.
对Lys72的结果表明,其pK对希夫基化学物质没有优化,这与之前的研究一致.

结论:

更多相关视频

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Published on: February 24, 2026

相关实验视频

Last Updated: Jul 16, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Methods to Identify the NMR Resonances of the ¹³C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Published on: February 24, 2026

[5-13C]氨酸方法是蛋白质定位研究的优越报告者,可以在更窄的pH范围进行可靠的测量.

在DNA聚合酶β中确定Lys72的pK值支持其在酶催化中的作用.

在TEM-1β-乳糖酶中对Lys73 pK进行了重新评估.