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相关概念视频

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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相关实验视频

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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南大洋的生物地质化学划分.

I Marinov1, A Gnanadesikan, J R Toggweiler

  • 1Atmospheric and Oceanic Sciences Program, Princeton University, Princeton, New Jersey 08540, USA. imarinov@mit.edu

Nature
|June 23, 2006
PubMed
概括

南大洋是南方的海洋.

科学领域:

  • 海洋学 海洋学 海洋学
  • 生物地质化学生物地质化学
  • 气候科学 气候科学

背景情况:

  • 南大洋的营养和碳循环显著影响全球二氧化碳 (CO2) 水平和生物生产.
  • 之前的模型表明,高度地区对于大气二氧化碳调节至关重要,营养利用与二氧化碳水平密切相关.

研究的目的:

  • 调查特定的南大洋地区是否对空海二氧化碳平衡和生物出口生产产生不同的影响.
  • 在南大洋内确定碳循环和生物出口的独特区域控制.

主要方法:

  • 利用海洋一般循环模型来模拟南大洋表面营养物质减少的增加.
  • 分析了模型对不同海洋地区营养物质可用性变化的反应.

主要成果:

  • 大气中的二氧化碳平衡主要由南极深水形成区域的生物和循环调节.
  • 全球生物出口产量主要由南极洲中介和模式水形成区域的生物和循环控制.

结论:

  • 南大洋的南极和南极以下地区之间存在生物地质化学分裂.
  • 这种分离表明有针对性的气候干预有可能明显地影响碳循环或生物生产.

更多相关视频

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相关实验视频

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