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用生物编码纳米粒子探针多重检测蛋白质癌症标记物

Savka I Stoeva1, Jae-Seung Lee, Jennifer E Smith

  • 1Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA.

Journal of the American Chemical Society
|June 29, 2006
PubMed
概括

研究人员创建了一个通用探针和纳米粒子标签,用于在低度下高度选择性地检测三种癌症蛋白标记物. 这种新的生物码放大方法可以进行多重分析,以改善疾病的诊断和监测.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...